Table 4.

Catalytic efficiencies of BdPTE mutants towards V-type nerve agents

kcat/KM (× 106 M−1 min−1)	BdPTE-1	BdPTE-2b	BdPTE-3	BdPTE-4	BdPTE-5b	BdPTE-6	BdPTE-7
V1	26.40 ± 0.24	146 ± 4.01	69.4 ± 1.56	49.9 ± 1.56	26.90 ± 0.45	17.20 ± 0.20	38.10 ± 0.36
V2	14.9 ± 0.39	87.6 ± 3.68	38.2 ± 0.51	23.6 ± 0.26	14.50 ± 0.12	9.94 ± 0.16	12.80 ± 0.11
V3	4.21 ± 0.17	16.1 ± 0.03	5.26 ± 0.50	3.05 ± 0.03	3.12 ± 0.09	2.17 ± 0.08	4.33 ± 0.14
V4 (VX)	11.30 ± 0.35	45.9 ± 0.76a	63.1 ± 0.24a	38.1 ± 0.36	11.80 ± 0.17	16.80 ± 0.47	20.70 ± 0.48
V5	4.41 ± 0.06	10.2 ± 0.03	5.43 ± 0.18	5.21 ± 0.11	1.71 ± 0.02	1.57 ± 0.07	3.45 ± 0.02
V6	5.61 ± 0.43	28.3 ± 0.34	21.8 ± 0.84	16.1 ± 0.04	4.74 ± 0.55	4.24 ± 0.05	9.62 ± 0.39
V7	2.74 ± 0.10	5.69 ± 0.03	2.73 ± 0.13	3.58 ± 0.03	1.18 ± 0.02	1.44 ± 0.04	4.03 ± 0.11
V8 (VR)	1.03 ± 0.004	5.68 ± 0.07	6.06 ± 0.05a	5.89 ± 0.26	21.40 ± 0.14	26.20 ± 0.98	22.20 ± 0.66
V9 (CVX)	2.00 ± 0.06	5.98 ± 0.19	6.49 ± 0.22	5.24 ± 0.24	10.60 ± 0.22	11.10 ± 0.34	9.60 ± 0.22
V10	0.30 ± 0.01	0.64 ± 0.01	0.66 ± 0.06	0.56 ± 0.01	2.01 ± 0.003	0.33 ± 0.006	0.90 ± 0.03

^aData from Job et al. (2020)

^bData from Escher et al. (2020)