Table 5.

Catalytic efficiencies of BdPTE mutants towards amiton analogues

kcat/KM (× 106 M−1 min−1)	BdPTE-1	BdPTE-2a	BdPTE-3	BdPTE-4	BdPTE-5a	BdPTE-6	BdPTE-7
A1	0.25 ± 0.005	0.97 ± 0.03	0.44 ± 0.02	0.66 ± 0.02	0.12 ± 0.002	0.15 ± 0.005	0.22 ± 0.01
A2 (amiton)	0.05 ± 0.001	0.22 ± 0.01	0.14 ± 0.02	0.20 ± 0.005	0.04 ± 0.001	0.03 ± 0.0004	0.08 ± 0.002
A3	0.02 ± 0.001	0.26 ± 0.004	0.02 ± 0.001	0.06 ± 0.003	0.01 ± 0.0003	0.02 ± 0.0004	0.05 ± 0.0004

^aData from Escher et al. (2020)