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. 2021 Jul 22;41(7):BSR20200533. doi: 10.1042/BSR20200533

Figure 4. miR-34a is negatively correlated with 5-Fu resistance and inhibits glycolysis.

Figure 4

(A) Comparison of miR-34a levels in cervical tumor tissues and matched normal tissues by qRT-PCR. (B) Expressions of miR-34a in CaSki parental and 5-Fu resistant cells detected by qRT-PCR. (C) miR-34a or negative control were transfected into CaSki and (D) SiHa cells, expressions of miR-34a were measured by qRT-PCR. (E) The CaSki and (F) SiHa cells without or with miR-34a overexpression were treated with 5-Fu at the indicated concentrations for 48 h, followed by MTT assay to examine the cell viabilities. (G) The glucose uptake and (H) lactate product were measured in CaSki and SiHa cells without or with miR-34a overexpression. (I) The mRNA expressions of glycolysis enzymes, HK2, PKM2 and LDHA were measured by qRT-PCR from CaSki cells without or with miR-34a overexpression. U6 was used as an internal control. All data were shown as mean ± S.D. *, P<0.05; **, P<0.01; ***, P<0.001.