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. 2021 Jul 22;11:15027. doi: 10.1038/s41598-021-94581-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NAG-1 overexpression attenuated high-fat diet/streptozotocin-induced diabetic dyslipidemia and hepatic steatosis in mice. The tissue sections were prepared and stained with hematoxylin and eosin (H&E). (A) Representative image of the H&E-stained section of the liver, iWAT, eWAT, and BAT. Scale bar = 50 µm. (B–D) Average lipid droplet area in the iWAT, eWAT, and BAT. (E) Histological staining of crown-like structure (CLS) in the eWAT. Scale bar = 50 µm. (F) Number of CLS per field (10 fields per group). Data are presented as mean ± standard error of mean. *p < 0.05 and ***p < 0.001, compared with the control group.