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. 2021 Jul 22;11:15027. doi: 10.1038/s41598-021-94581-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of NAG-1 on the mRNA expression level of adipocytokines. Adiponectin and leptin mRNA expression levels in the muscle tissue, inguinal white adipose tissue, and brown adipose tissue were determined using quantitative real-time polymerase chain reaction. The expression levels of target genes were normalized with those of Gapdh. Data are presented as mean ± standard error and were analyzed using the Student’s t-test. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the control group (n = 3).