Table 1.
PCR primers used in this study.
| Forward primer (5′→3′) | Reverse primer (5′→3′) | |
|---|---|---|
| Adiponectin | ATCTGGAGGTGGGAGACCAA | GGGCTATGGGTAGTTGCAGT |
| Leptin | AGGTAGGGATGGGTAGAGCC | GTGTGCTGCTTGGGAGTTTC |
| GLUT4 | GGTGTGGTCAATACGGTCTTCAC | AGCAGAGCCACGGTCATCAAGA |
| PI3K | CAAACCACCCAAGCCCACTACT | CCATCAGCAGTGTCTCGGAGTT |
| Ptpn1 | GCGCTTCTCCTACCTGGCTGTCAT | ACGTGCTCGGGTGGAAGGTCTA |
| IRS-1 | TGTCACCCAGTGGTAGTTGCTC | CTCTCAACAGGAGGTTTGGCATG |
| Akt | GGACTACTTGCACTCCGAGAAG | CATAGTGGCACCGTCCTTGATC |
| GSK3β | CATAGTGGCACCGTCCTTGATC | CCAACTGATCCACACCACTGTC |
| PPARγ | GTACTGTCGGTTTCAGAAGTGCC | ATCTCCGCCAACAGCTTCTCCT |
| FOXO1 | CTACGAGTGGATGGTGAAGAGC | CCAGTTCCTTCATTCTGCACTCG |
| AS160 | GCCAACAGTCTTGCCTCAGAGA | CGTCTTCGGAACTGTGGAGAGT |
| RPTOR | CTTCCTATCCGTCTTGGCAGAC | CTCCAGACAGATGGCAATCAGG |
| RICTOR | CAGTGTGAGGTCCTTTCCATCC | GCCATAGATGCTTGCGACTGTG |
| UCP1 | TTTTGTTCTTGCACTCACGCC | CCCATGGTGGGTTGCACTTC |
| PRDM16 | CCACCAGCGAGGACTTCAC | GGAGGACTCTCGTAGCTCGAA |
| Cidea | CAGTGATTTAAGAGACGCGG | TCTGCAATCCCATGAATGTC |
| Dio2 | ATGCTGACCTCAGAAGGGCT | ACACTGGAATTGGGAGCATC |
| FGF21 | ATCAGGGAGGATGGAACAGTGG | AGCTCCATCTGGCTGTTGGCAA |
| NLRP3 | TGCTCTTCACTGCTATCAAGCCCT | ACAAGCCTTTGCTCCAGACCCTAT |
| IL-18 | TGGTTCCATGCTTTCTGGACTCCT | TTCCTGGGCCAAGAGGAAGTGATT |
| IL-1β | TGGACCTTCCAGGATGAGGACA | GTTCATCTCGGAGCCTGTAGTG |
| ASC | CTGCTCAGAGTACAGCCAGAAC | CTGTCCTTCAGTCAGCACACTG |
| Caspase-1 | GGCACATTTCCAGGACTGACTG | GCAAGACGTGTACGAGTGGTTG |
| GAPDH | CATCACTGCCACCCAGAAGACTG | CATCACTGCCACCCAGAAGACTG |