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. 2021 Jul 22;11:15000. doi: 10.1038/s41598-021-94603-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the yield of purified EVs from serum between Tim4- and anti-CD9 antibody-based methods. Serum of normal donors (n = 6, 50 µL) was incubated with magnetic beads on which Tim4 or anti-CD9 antibody (HI9a or A100-4) was immobilized. After washing, bound EVs were released from Tim4 and anti-CD9 antibody by adding EDTA and glycine–HCl, respectively. Then, the purified EVs were subjected to bead-based sandwich ELISA for exosomal surface markers CD9 (a) and CD63 (b). The signal intensity is expressed as a ratio to that obtained with Tim4. Data are shown by mean ± SD.