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. 2021 Jul 22;11:15000. doi: 10.1038/s41598-021-94603-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measurement of the PSMA-EV levels in serum of mouse xenograft model for PC. The C4-2B PC cells were injected into the subcutaneous space of athymic nude mice. After the formation of tumor, blood was collected and the xenograft tissues were removed from control (n = 10) and xenograft mice (n = 21). (a) The tumor weight was measured. (b) The PSMA-EV levels in serum was determined after purification of EVs from serum by Tim4. Data are shown in the 'box and whiskers' graph with interquartile range (IQR: box), median (thick vertical line in the box) and 1.5 IQR (whiskers). x denotes outliers.