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. 2021 Jul 22;11:15000. doi: 10.1038/s41598-021-94603-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measurement of the PSMA-EV levels in serum of PC patients. The PSMA-EV levels in serum was determined after purification of EVs from serum by Tim4. Patients with therapy-naïve PC and CRPC were analyzed. Comparisons were made between non-metastatic (n = 55) and metastatic patients (n = 11) (a) and among organ-confined PC patients (≤ T2, n = 39), locally-advanced PC patients without metastasis (≥ T3, n = 16) and CRPC patients (n = 16) (b). Data are shown in the 'box and whiskers' graph with interquartile range (IQR: box), median (thick vertical line in the box) and 1.5 IQR (whiskers). x denotes outliers.