Fig. 4. Hpr1 and Sen1 degron strains accumulate H2AP in S-phase.
a Immunoblotting with αH2AP of WT, hpr1-aid, and sen1-aid cells in asynchronous (A), G1, S-phase, and G1-phase after one complete cell cycle (G12) cultures. AtTIR1-9Myc was used as loading control. b Immunoblotting quantification (means +/− SEM) of protein levels normalized to the WT (n = 3 biologically independent samples). c H2AP ChIP analysis at the GCN4 and PDC1 genes in WT, hpr1-aid, and sen1-aid strains with or without RNH1. Data are presented as mean values +/− SEM (n = 7 biologically independent experiments). The P values are calculated by the two-tailed unpaired Student t-test. A diagram of the experiment is represented above each experiment.