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. 2021 Jul 22;12:4462. doi: 10.1038/s41467-021-24755-9

Fig. 3. Tfam deficiency in γδT17 cells cause small intestine lengthening.

Fig. 3

a Picture of the small intestine of 8-week-old littermate Tfam+/+ Rorc-cre and Tfamfl/flRorc-cre mice. Representative data of three independent experiments. b Small intestine length of mice with indicated genotypes at different ages (week 3, P = 0.8542; week 6, P = 0.056; week 8, *P = 0.03853; week 12, ****P < 0.0001). Ctr, n = 5 for 3-week-old and 12-week-old age, n = 4 for 6-week-old age, n = 3 for 8-week-old age, Tfamfl/flRorc-cre, n = 4. Compiled data from three independent experiments. c Hematoxylin and eosin (H&E) staining of the small intestine from 12-week-old mice with indicated genotypes. Representative data of two independent experiments. Scale bar, 100 µm. d Histology score of the small intestine (n = 3 for each group) (*P = 0.0135). Each dot represented the data from an individual mouse. Compiled data from one experiment. e RORγt and CD45.1 expression in Lin– cells (upper panel) or total γδT cells (lower panel) measured by flow cytometry in Ctr or Tfamfl/flRorc-cre mice with or without CD45.1+ wild-type (WT) bone marrow (BM) transfer. Representative data of two independent experiments. f Percentages of γδT17 cells in total γδT cells (Ctr vs. Tfamfl/flRorc-cre, ****P < 0.0001; Ctr + CD45.1+ BM vs. Tfamfl/flRorc-cre  + CD45.1+ BM transfer, ***P = 0.0002). g Percentages of ILC3s in Lin– cells (Ctr vs. Tfamfl/flRorc-cre, ****P < 0.0001; Ctr + CD45.1+ BM vs. Tfamfl/flRorc-cre + CD45.1+ BM, *P = 0.0382; Tfamfl/flRorc-cre vs. Tfamfl/flRorc-cre + CD45.1+ BM, **P = 0.0059). h Percentages of CD45.1+ ILC3s in total ILC3s (Ctr + CD45.1+ BM vs. Tfamfl/flRorc-cre + CD45.1+ BM, ***P = 0.0002). i Picture of the small intestine. j Small intestine length. Compiled data from two independent experiments in f–h, j (Ctr vs. Tfamfl/flRorc-cre, **P = 0.0037; Ctr + CD45.1+ BM vs. Tfamfl/flRorc-cre + CD45.1+ BM, **P = 0.0014) (n = 4 for Ctr with or without BM transfer groups and n = 5 for Tfamfl/flRorc-cre mice with or without BM transfer groups). k Picture of the small intestine of mice with indicated genotypes. Five-week-old age mice were treated with tamoxifen (2 mg/mouse by intraperitoneal injection) daily for 5 days. Data were collected 3 weeks after the last tamoxifen injection. l Small intestine length. Compiled data from three independent experiments (n = 6 for Ctr, n = 7 for Tfamfl/flTcrdCreER mice). Ctr included Tfam+/+, Tfamfl/+, Tfamfl/fl, Tfam+/+Rorc-cre, and Tfamfl/+Rorc-cre mice in b, d, f–h, j (**P = 0.0060). Ctr included Tfam+/+TcrdCreER and Tfamfl/+TcrdCreER mice in l. Data are shown as mean ± SD in b, d, f–h, j, l.