Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 22;12:4462. doi: 10.1038/s41467-021-24755-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Analysis of Dclk1 and Trpm5 expression by qRT-PCR in enteroid culture (n = 3) (mean ± SD) (Dclk1, ***P = 0.0008; Trpm5, ****P < 0.0001). Representative data of three independent experiments. b DCLK1 expression in enteroid culture with indicated treatment (IL-13 (50 ng/ml), IL-17A (100 ng/ml), and/or IL-22 (5 ng/ml)) measured by flow cytometry. Representative data of two independent experiments. c Percentages of DCLK1⁺ cells in EpCAM⁺ cells (mean ± SD) (n = 3) (none vs. IL-13, ****P < 0.0001; IL-13 vs. IL-13 + IL-22, **P = 0.0045). Compiled data from one experiment. d Confocal analysis of DCLK1 expression in enteroid culture with indicated treatment. Representative data of two independent experiments. Green, DCLK1. Blue, DAPI. Scale bar: 50 µm. e Left panel, picture of the small intestine of 8-week-old littermate Il22^Cre/+ and Il22^Cre/Cre mice ⁽*P = 0.0223). Representative data of two independent experiments. Right panel, small intestine length in mice with indicated genotypes (n = 3). Compiled data from two independent experiments. f IL-4, IL-5, and IL-13 expression in ILC2s or CD4⁺ T cells by flow cytometry from the small intestine of 8-week-old Il22^Cre/+ and Il22^Cre/Cre mice (n = 3) (ILC2: IL-4, **P = 0.0076; IL-5, **P = 0.0024; IL-13, *P = 0.0245; CD4⁺T: IL-4, P = 0.8613; IL-5, P = 0.4343; IL-13, P = 0.2181). Compiled data from two independent experiments. g Analysis of Dclk1, Trpm5, Gnat3, and Il25 expression by qRT-PCR in small intestine tissues of 8-week-old Il22^Cre/+ and Il22^Cre/Cre mice (n = 3) (Dclk1, ****P < 0.0001; Trpm5, **P = 0.0023; Gnat3, ****P < 0.0001; Il25, **P = 0.0062). Representative data of two independent experiments. h, i Tfam^fl/flRorc-cre mice at the age of 6 weeks old received hydrodynamic tail vein injection of either empty or IL-22 vectors. Mice were sacrificed at 10 weeks of age and small intestine tissues were harvested. h qRT-PCR gene expression analysis of small intestine tissue IL-22-target gene Reg3g, type 2 cytokine genes Il4, Il5, and Il13, and tuft cell-associated genes Dclk1, Trpm5, Gnat3, and Il25 (n = 3) (Reg3g, ***P = 0.0001; Il4, *P = 0.0139; Il5, ***P = 0.0004; Il13, ***P = 0.0001; Dclk1, ****P < 0.0001; Trpm5, ****P < 0.0001; Gnat3, **P = 0.0011; Il25, **P = 0.0063). Representative data of three independent experiments. i Top panel, picture of the small intestine length. Bottom panel, quantification of the small intestine length (n = 4 for empty group and n = 3 for IL-22 treatment group) (***P = 0.0009). Compiled data from one experiment. Data are shown as mean ± SD in a, c, e–i.