Table 1.
SYNB1618 PAL activity model parameters.
| Parameter/state variable | Description | Value (Range) | Initial Value | Units |
|---|---|---|---|---|
| Phe | Phe concentration | - | 40 | μmol/mL |
| TCA | TCA concentration | - | 0 | μmol/mL |
| t | Time | - | - | min |
| Cells | SYNB1618 cell density | - | - | CFU/mL |
| KmPAL | Michaelis–Menten constant for SYNB1618 with Phe | 0.0184 (0.005–0.0442) | - | μmol/mL |
| VmaxPAL | Maximum rate of TCA production by SYNB1618 PAL activity | 0.0162 (0.0154–0.0171) | - | μmol/(min*109 CFU) |
| KipH | Inhibition constant for PAL due to pH | (0.0–1.0) | - | Unitless |
| KiTCA | Inhibition constant for PAL due to feedback inhibition by TCA | (0.0–1.0) | - | Unitless |
| pH | pH | (3.0–8.0) | - | Unitless |