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. 2021 Jul 22;11:14965. doi: 10.1038/s41598-021-94017-7

Figure 2.

Figure 2

Sequencing data of pigs generated by intracytoplasmic microinjection of the CRISPR/Cas9 construct targeting the porcine TRDC-locus. Sequencing results confirmed the PCR results (Fig. 1) that pigs 695-2, -3 and pigs 696-1, -5, -6, -7 carry a homozygous knockout of the TRDC locus consisting of a clear 40 bp deletion. For each piglet, six samples were sequenced. None of the piglets were monoallelic knockouts (the binding sequences of the guide RNAs are indicated by green arrows).