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. 2021 Jul 22;11:14951. doi: 10.1038/s41598-021-94544-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GATA6 IHC expression levels. No or weak GATA6 IHC expression (scores 0–1) without digital assistance (A,C) and with digital assistance (B,D) showing tumor as predominantly blue. Moderate GATA6 IHC expression (score 2) without digital assistance (E) and with digital assistance (F) showing tumor as predominantly yellow. (D) Strong GATA6 IHC expression (score 3) without digital assistance (G) and with digital assistance (H) showing tumor as predominantly red (≥ 50% of tumor cells). (E) Very strong GATA6 IHC expression (score 4) without digital assistance (I) and with digital assistance (J) showing tumor as nearly all red (≥ 90% of tumor cells). GATA6 IHC expression levels were regrouped into low (0–2) and high (3–4) groups to assess for an association with clinical outcome.