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. 2021 Jul 22;12:4469. doi: 10.1038/s41467-021-24715-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Side view of the density map of the HBB complex (EMD-30409) without applying any symmetry (C1). OM, PG, IM refers to the outer membrane, peptidoglycan layer and inner membrane, respectively. A vertical slice of the LP ring and rod is shown enlarged on the right. The atomic models of the L ring (purple) and the P ring (cyan) are superimposed to the density map. The yellow and red circles indicate the positions of Lys-63 and Lys-95 of FlgI. b The density maps of the L ring (purple) and the P ring (cyan) after applying C26 symmetry (EMD-30398). c The atomic models of FlgH and FlgI in Cα ribbon representation in a vertical slice of the LP ring. The L ring has a three-layered structure (inner layer, light green; middle layer, cyan; outer layer, pink), and the P ring has a two-layered structure (inner ring, light yellow; outer ring, blue). d The atomic models of FlgH and FlgI (PDB ID: 7CLR) in Cα ribbon representation. FlgH is constituted from three parts: the inner layer domain; the middle layer domain; and the outer layer chain. FlgI is constituted from three domains: FlgI-OR (cyan); FlgI-IR_U (light green); and FlgI-IR_L (pink). Lys-63, Lys-95 and the P ring loop are located within the FlgI-IR_U domain.