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. 2021 Jul 22;12:4478. doi: 10.1038/s41467-021-24717-1

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative epi-fluorescence images of doublecortin (DCX) immunoreactivity (green) at the hippocampal dentate gyrus two days after acute high-dose radiation (AH), “Pulsed conditioning” (PC), double (2×) PC, triple (3×) PC, quadruple (4×) PC, “Free moving conditioning” (FC), or no radiation (Con), or at 8 weeks after FC radiation (8 w after FC). Blue: DAPI. b Quantification of DCX-positive cells under different conditions (n = 3 mice for each group). Con vs. PC, p > 0.9999; Con vs. 2× PC, p = 0.531; Con vs. 3× PC, *p = 0.0488; Con vs. 4× PC, *p = 0.0102; Con vs. FC, ****p < 0.0001; Con vs. AH, ****p < 0.0001; Con vs. 8 w after FC, ***p = 0.0001; FC vs. 8 w after FC, p > 0.9999; Bonferroni’s multiple comparison test, two-sided. c Quantification of total bone marrow cells three days after X-irradiation (Con, n = 9 mice; PC, n = 5 mice; 2× PC; n = 4 mice; 3× PC, n = 4 mice; 4× PC, n = 4 mice; FC, n = 5 mice; AH, n = 5 mice). PC, ****p < 0.0001; 2× PC, p = 0.987; 3× PC, p = 0.9998; 4× PC, p = 0.887; FC, ****p < 0.0001; AH, ****p < 0.0001; Dunnett’s multiple comparison tests vs. Con, two-sided. N.S.: not significant. Open circles indicate individual data. Values are mean ± SEM.