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. 2021 Jul 22;12:4458. doi: 10.1038/s41467-021-24788-0

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Embryonic Ppy-expressing cell labeling strategy. DOX doxycycline, P30 postnatal day 30. b The proportion of adult YFP-labeled Ppy-expressing cells is maintained in the different DOX administration periods. DOX E7.5 to P30, analysis P30 (black; n = 4 mice, 1842 Ppy+YFP+ out of 2131 Ppy+ cells scored); DOX E7.5 to E19.5, analysis P30 (dark gray, n = 7 mice, 3111 Ppy+YFP+ out of 3604 Ppy+ cells scored); DOX E7.5 to E19.5, analysis 9-month-old (light gray, n = 4 mice, 2241 Ppy+YFP+ out of 2565 Ppy+ cells scored). Data are shown as mean ± s.e.m; two-tailed Mann–Whitney test. P values: DOX E7.5 to P30, analysis P30 vs DOX E7.5 to E19.5, analysis P30 = 0.9273; DOX E7.5 to P30, analysis P30 vs DOX E7.5 to E19.5, analysis long term = 0.6857. c Percentage of adult hormone⁺ cells (Gcg⁺, Sst⁺ or Ins⁺) labeled with YFP when DOX was given during embryogenesis. 21.1 ± 0.0%, 10.8 ± 0.0% and 6.1 ± 0.0% of Gcg⁺, Sst⁺ and Ins⁺, respectively, were YFP-traced. The percentage of YFP-labeled Ppy+ cells is taken for reference from panel b. Data are shown as mean ± s.e.m; n = 7 mice; 810 YFP+Gcg+ out of 4196 Gcg+, 285 YFP+Sst+ out of 2669 Sst+ and 1306 YFP+Ins+ out of 20753 Ins+ cells scored. d Immunofluorescence of YFP (green)-traced cells co-expressing Glucagon (red, left panel), Somatostatin (red, middle panel) and Insulin (red, right panel) with Ppy (cyan) at P30. Scale bar: 20 μm (10 μm in insets). Region of the pancreas: Ventral. Source data are provided as Source Data file (Supplementary Table e).