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. 2021 Jul 22;11:15039. doi: 10.1038/s41598-021-94469-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric detection of regulatory T cells. (A) The lymphocyte population was defined on forward, and side scatter histogram (R1). (B) The expression of CD4 on the lymphocytes population was detected, then CD4⁺ cells were gated for further analysis of CD25. (C) Three gates were drown to define CD4⁺CD25^- cells, CD4⁺CD25^+low cells and CD4⁺CD25^+high cells. (D) The percentage of CD4⁺CD25^+highFoxp3⁺cells (regulatory T cells) was then assessed.