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. 2021 Jul 22;11:15039. doi: 10.1038/s41598-021-94469-x

Figure 2.

Figure 2

Flow cytometric detection of myeloid-derived suppressor cells. (A) HLA-DR negative cells (R1) were selected from HLA-DR and side scatter histogram. (B) HLA-DR negative cells were assessed for their expression of CD33 and CD11b to detect total myeloid-derived suppressor cells (MDSCs: HLA-DR-CD33+CD11b+). (C,D) Total myeloid-derived suppressor cells were assessed for their expression of CD15 and CD14 to detect monocytic myeloid-derived suppressor cells (MO-MDSCs: HLA-DR-CD33+CD11b+CD14+), and polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs “previously G-MDSCs”: HLA-DR-CD33+CD11b+ CD15+).