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. 2021 Jul 22;12:4474. doi: 10.1038/s41467-021-24734-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a (Left) Representative fluorescent photomicrographs of liver sections of ND, HFHC, and RES (3 weeks) mice stained with 4′,6-diamidino-2-phenylindole (DAPI: blue), anti-CD8 Ab (green), and anti-desmin Ab (red). Insets show higher magnification. Scale bars: 50 µm. (Right) Count of CD8⁺ T cells co-localized with HSCs in unit area of liver section　(n = 4 mice per group). b, c Ccl3, Ccl4, and Ccl5 mRNA levels in sorted CD8 T cell subsets (b) (n = 3 mice per group), and isolated hepatocytes (c) (n = 4 mice per group). d Frequency of CCR5⁺ cells in CD45⁺ liver MNCs and HSCs of the indicated groups (n = 3 mice per group). e Cell numbers of migrated HSCs in lower compartment containing supernatants of CD3/28-stimulated CD8⁺ T cell subset with or without CCR5 inhibitor, Marovenic (MVC) (left; HSCs and CD8⁺ T cells isolated from RES mice, right; isolated from HFHC mice, n = 3 biologically independent samples per group). f Study design: RES mice were intravenously treated with vitamin A-control siRNA liposomes (RES group) or vitamin A-Ccr5 siRNA liposomes (RES + HSC specific CCR5KD group) twice per week starting from 2 weeks following the diet switch to 5 weeks (n = 6 mice per group). g Ccr5 mRNA levels in isolated HSCs, Kupffer cells, and hepatocytes of the indicated groups. h (left) Representative fluorescent photomicrographs of liver sections stained with DAPI (blue), anti-desmin Ab (red), and anti-CD8 Ab (green). Scale bars: 50 µm. (right) Count of CD8⁺ T cells co-localized with HSCs in unit area of liver section. i Serum ALT levels. j Hydroxyproline levels. k Representative photomicrographs (left) and positive area (right) of Sirius Red staining of liver sections. Scale bars: 200 µm. l Fibrosis associated genes (Col1a1, Col1a2, Acta2, Timp1, Desmin, Spp1) mRNA levels. m HSCs count in unit area of liver section. n Number of CD45⁺CD11b⁺CD11c⁻ macrophages in CD45⁺ liver MNCs. o (left) Representative fluorescent photomicrographs of liver sections stained with DAPI (blue), anti-desmin Ab (green), and TUNEL (pink). Scale bars: 50 µm. (right) Count of TUNEL positive HSCs in unit area of liver section. Data are presented as mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons post-hoc test (a–c, e) or two-sided unpaired Student’s t test (g–o) was applied. Source data are provided as a Source data file.