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. 2021 Jul 22;12:4474. doi: 10.1038/s41467-021-24734-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a (left) Representative fluorescent photomicrographs of liver sections from ND, HFHC, and RES (3 weeks) mice stained with anti-desmin Ab (green) and TUNEL (pink). Scale bars: 50 µm. (right) Count of TUNEL positive HSCs in unit area of liver section (n = 4 mice per group). b, c Percent cytotoxicity of HSCs by CD8 Trm (red) or CD8 T naive (blue) isolated from RES 5 weeks mice (b) or HFHC mice (c) (n = 3 biologically independent samples per group). d Fasl mRNA levels in sorted CD8⁺ T cell subsets (n = 3 mice per group). e Count of FasL positive CD8⁺ T cells in unit area of liver section (n = 4 mice per group). f Percent cytotoxicity of HSCs co-cultured with CD8⁺ Trm cells in the presence of the indicated apoptosis inhibitors for 8 h (n = 3 biologically independent samples per group). g Fas mRNA levels in isolated HSCs and hepatocytes of the indicated mice (n = 4 mice per group). h (left) Representative fluorescent photomicrographs of liver sections stained with DAPI (blue), anti-desmin Ab (green), and anti-Fas (red). Scale bars: 50 µm. (right upper) Count of Fas positive HSCs in unit area of liver section (n = 4 mice per group). (right lower) Positive area of Fas positive HSCs in unit area of liver section. i Study design: resolution induced mice were intraperitoneally injected with anti-FasL antibody (RES + FasL neutralization group) or the isotype control (RES group) once every 3 days for 3 weeks starting at week 2 following diet switch (n = 7 mice per group). j Serum ALT levels. k Hydroxyproline levels. l Representative photomicrographs (left) and positive area (right) of Sirius Red staining of liver sections. Scale bars: 200 µm. m Fibrosis associated genes (Col1a1, Col1a2, Acta2, Timp1, Desmin, Spp1) mRNA levels. n HSCs count in unit area of liver section. o Number of CD45⁺CD11b⁺ CD11c⁻ macrophages in CD45⁺ liver MNCs. p (left) Representative fluorescent photomicrographs of liver sections stained with DAPI (blue), anti-desmin Ab (green), and TUNEL (pink). Scale bars: 50 µm. (right) Count of TUNEL positive HSCs in unit area of liver section. Data are presented as mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons post-hoc test (a, d–h) or two-sided unpaired Student’s t test (j–p) was applied. Source data are provided as a Source data file.