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. 2021 Jul 22;11:14961. doi: 10.1038/s41598-021-94095-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PREDICT96-ALI platform. (A) Schematics illustrating the configuration of the PREDICT96-ALI platform, with the overall standard 384-well plate layout shown at the left. Detail for an individual airway model element of the array is shown at right, where the top chamber appears as an oval region and the underlying bottom chamber an inverse U-shaped channel with inlet and outlet ports. (B) Exploded view stack of patterned polymer films used to create the structure, which is fabricated at the plate level but is shown at the level of an individual model. (C) Photographs of the upright view (left), inverted view (right) and overhead view (bottom), with magnified view of an individual model within the PREDICT96-ALI platform configuration.