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. 2021 Jul 22;11:14961. doi: 10.1038/s41598-021-94095-7

Figure 5.

Figure 5

Infection kinetics of IAV-inoculated PREDICT96-ALI airway tissue. RT-qPCR analyses for viral copies in the apical wash of PREDICT96-ALI airway tissue at 24 and 48 h p.i. (A) PREDICT96-ALI airway tissue inoculated with A/California/04/09 H1N1 and monitored for an increase in viral load at 24 h intervals with a statistically significant increase in viral copies at 48 h p.i. compared to 24 h p.i. for the MOI 1 condition. (B) PREDICT96-ALI airway tissue inoculated with A/Hong Kong/8/68 H3N2 and monitored for an increase in viral load at 24 h intervals with a statistically significant increase in viral copies at 48 h p.i. compared to 24 h p.i. for the MOI 1 condition. Statistical significance: ***p ≤ 0.001 and ****p ≤ 0.0001. N = 2 independent experiments with data presented from one representative experiment. Per experiment, N = 3–4 tissue replicates per donor, time-point and condition. Donor B showcased as representative. Replicates that did not meet the minimum signal intensity after 45 PCR cycles are displayed along the x-axis.