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. 2021 Jul 22;11:14961. doi: 10.1038/s41598-021-94095-7

Figure 6.

Figure 6

Infection Kinetics of IAV-inoculated PREDICT96-ALI Airway Tissue in Response to Oseltamivir. RT-qPCR analyses for viral copies of the apical wash of PREDICT96-ALI airway tissue at 24 and 48 h p.i. in absence (blue, vehicle control) or presence (red = 0.01 µM, green = 0.1 µM, violet = 1 µM, black = 10 µM) of oseltamivir (Tamiflu) for two donors labeled donor B (A) and donor C (B). PREDICT96-ALI airway tissue inoculated with A/Hong Kong/8/68 H3N2 at MOI = 0.1, and monitored for viral load over 24 h intervals up to 48 h p.i. with a marked decrease in viral copies in response to oseltamivir dose at both time points. N = 2 independent experiments with data presented from one representative experiment. Per experiment, N = 3–4 tissue replicates per donor, time-point and condition. Replicates that did not meet the minimum signal intensity after 45 PCR cycles are displayed along the x-axis.