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. 2021 Jul 22;11:14961. doi: 10.1038/s41598-021-94095-7

Figure 8.

Figure 8

Infection kinetics of HCoV-NL63-inoculated PREDICT96-ALI airway tissue. RT-qPCR analyses for viral copies in the apical wash of PREDICT96-ALI airway tissue at 0, 48 and 96 h p.i. for two human donors showing unique infection kinetic profiles. (A) PREDICT96-ALI donor A airway tissue inoculated with HCoV-NL63 and monitored for an increase in viral load at 48 h intervals with a statistically significant increase in viral copies at 48 and 96 h p.i compared to 0 h p.i. for the MOI 0.1 and 0.5 conditions. Statistical significance: ****p ≤ 0.0001. (B) PREDICT96-ALI donor B airway tissue inoculated with HCoV-NL63 and monitored for an increase in viral load at 48 h intervals with a statistically significant increase in viral copies at 96 h p.i compared to 48 and 0 h p.i. for MOI 0.05, 0.1, and 0.5, as well as a statistically significant increase in viral copies at 48 h p.i. compared to 0 h p.i. for MOI 0.1 and 0.5 conditions. Transient, non-productive viral infection detected at 48 h p.i. in mock condition (0 MOI), is likely the result of technical handling by the experimental operator. Statistical significance: **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001. N = 2 independent experiments with data presented from one representative experiment. Per experiment, N = 3–4 tissue replicates per donor, time-point and condition. Replicates that did not meet the minimum signal intensity after 45 PCR cycles are displayed along the x-axis.