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. 2021 Jul 22;11:14961. doi: 10.1038/s41598-021-94095-7

Figure 9.

Figure 9

Infection of coronavirus strains in PREDICT96-ALI model. RT-qPCR analyses for viral copies of HCoV-NL63 (A,C) and HCoV-OC43 (B,D) in the apical wash of PREDICT96-ALI airway tissue model from a single donor, measured over 12 days across three different MOIs (0, 0.05, 0.5). (A) PREDICT96-ALI airway tissue inoculated with HCoV-NL63 and monitored for an increase in viral load at 48 h intervals with a statistically significant increase in viral copies at 48 and 96 h p.i. compared to 0 h p.i. for the MOI 0.05 condition, as well as a statistically significant increase at 48 h p.i. compared to 0 h p.i. for the MOI 0.5 condition. (B) PREDICT96-ALI airway tissue inoculated with HCoV-OC43 and monitored for an increase in viral load at 48 h intervals with a statistically significant increase in viral copies at 48 and 96 h p.i. compared to 0 h p.i. for the MOI 0.5 condition. Statistical significance: *p ≤ 0.05 and **p ≤ 0.01. N = 4–5 tissue replicates per donor, time-point and condition. Replicates that did not meet the minimum signal intensity after 45 PCR cycles are displayed along the x-axis. Viral copy number versus day post-infection shown for HCoV-NL63 (C) and HCoV-OC43 (D) shown for each MOI as indicated in the legends. Transient, non-productive viral infection detected as early as 48 h p.i. and as late as 144 h p.i. in mock condition (0 MOI), is likely the result of technical handling by the experimental operator.