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. 2021 Jul 22;11:14997. doi: 10.1038/s41598-021-94646-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adult individuals and larval state of B. wellingtonensis show a unique transcriptional signature. (A) Principal component analysis of the 21 RNAseq sample shows clustering into adult and larval groups. Upper circles show larval samples: EAL (red), AL (black), PLTIII (green) and PLTI (yellow). Lower circles show adult samples: FTIII (pink), FTI (lilac), and M (calypso). (B) Volcano plot comparison of differential gene expression between larvae and adults. Red dots highlight differentially expressed genes with threshold FDR < 0.01 and Log2 Fold Change [≥ 1]. The black zone indicates the number of transcripts that do not show significant differential expression.