Fig. 1. Previously investigated 6mA and 5mC contents throughout the fungal phylogeny.

A phylogeny of the fungal kingdom showing that there was a shift in the functional methylome during the divergence of EDF and Dikarya from high 6mA and low 5mC contents in EDF to to high 5mC and low 6mA contents in Dikarya. However, the arbuscular mycorrhizal fungi (Glomeromycotina) have not been previously investigated. *Represents species that do not follow the general pattern of methylation in EDF. In these species, 6mA is low and appears not to have the same functional significance as in EDF as it does not occur in ApT motifs, which are important for ensuring active gene transcription.