Table 2.
Annotated genes encoding lignocellulose-degrading enzymes in B. paralicheniformis CCMM B940
| Enzymes | Accession number | Activity | CAZyme family | Reference genes | Identity (%) | Subject sequence length (bp) | Alignment length (bp) | |
|---|---|---|---|---|---|---|---|---|
| Accession number | Species | |||||||
| Hemicellulose related | EOOONOAK_01749 | Aryl-phospho-beta-d-glucosidase BglC | GH1 | ARA87766.1 | Bacillus paralicheniformis MDJK30 | 99.78 | 472 | 472 |
| EOOONOAK_03335 | Aryl-phospho-beta-d-glucosidase BglH | GH1 | AGN35231.1 | Bacillus paralicheniformis ATCC 9945a | 100 | 100 | 100 | |
| EOOONOAK_03002 | Aryl-phospho-beta-d-glucosidase BglC | GH1 | AJO16530.1 | Bacillus paralicheniformis BL-09 | 100 | 491 | 478 | |
| EOOONOAK_01773 | Aryl-phospho-beta-d-glucosidase BglH | GH1 | QEO05069.1 | Bacillus paralicheniformis A4-3 | 99.57 | 469 | 469 | |
| EOOONOAK_03715 | Endo-1,4-beta-xylanase A | GH11 | QEO05734.1 | Bacillus paralicheniformis A4-3 | 100 | 213 | 213 | |
| EOOONOAK_02553 | Beta-glucanase | GH16 | ARA86799.1 | Bacillus paralicheniformis MDJK30 | 99.59 | 243 | 243 | |
| EOOONOAK_03322 | Mannan endo-1,4-beta-mannosidase | GH26 | QFY39913.1 | Bacillus paralicheniformis FA6 | 98.89 | 360 | 360 | |
| EOOONOAK_03205 | Beta-hexosaminidase | GH3 | QEO05378.1 | Bacillus paralicheniformis A4-3 | 99.69 | 644 | 643 | |
| EOOONOAK_02186 | Glucuronoxylanase XynC | GH30 | AJO20016.1 | Bacillus paralicheniformis BL-09 | 100 | 420 | 420 | |
| EOOONOAK_01418 | Oligosaccharide 4-alpha-d-glucosyltransferase | GH31 | QFY38704.1 | Bacillus paralicheniformis FA6 | 99.75 | 802 | 802 | |
| EOOONOAK_02225 | Alpha-xylosidase | GH31 | AJO19971.1 | Bacillus paralicheniformis BL-09 | 99.61 | 769 | 769 | |
| EOOONOAK_02112 | Levanbiose-producing levanase | GH32 | ARA87316.1 | Bacillus paralicheniformis MDJK30 | 99.8 | 515 | 495 | |
| EOOONOAK_01584 | Sucrose-6-phosphate hydrolase | GH32 | AJO20434.1 | Bacillus paralicheniformis BL-09 | 99.37 | 478 | 478 | |
| EOOONOAK_01729 | Sucrose-6-phosphate hydrolase | GH32 | AJO20595.1 | Bacillus paralicheniformis BL-09 | 98.98 | 492 | 492 | |
| EOOONOAK_00085 | Levanase | GH32CBM66 | QFY38018.1 | Bacillus paralicheniformis FA6 | 100 | 677 | 677 | |
| EOOONOAK_02224 | Hypothetical protein | GH3CBM6 | AJO19972.1 | Bacillus paralicheniformis BL-09 | 100 | 980 | 980 | |
| EOOONOAK_03904 | Beta-galactosidase BglY | GH42 | ARA84401.1 | Bacillus paralicheniformis MDJK30 | 99.42 | 690 | 690 | |
| EOOONOAK_04173 | Beta-galactosidase YesZ | GH42 | QFY39293.1 | Bacillus paralicheniformis FA6 | 100 | 665 | 665 | |
| EOOONOAK_01837 | Beta-galactosidase GanA | GH42 | QFY40673.1 | Bacillus paralicheniformis FA6 | 99.56 | 684 | 684 | |
| EOOONOAK_03858 | Beta-xylosidase | GH43 | ARA84797.1 | Bacillus paralicheniformis MDJK30 | 99.62 | 532 | 532 | |
| EOOONOAK_03859 | Non-reducing end alpha-l-arabinofuranosidase | GH43 | ARA84798.1 | Bacillus paralicheniformis MDJK30 | 99.03 | 515 | 515 | |
| EOOONOAK_03489 | Extracellular endo-alpha-(1- > 5)-l-arabinanase 1 | GH43 | ARA85201.1 | Bacillus paralicheniformis MDJK30 | 100 | 316 | 316 | |
| EOOONOAK_00089 | Beta-xylosidase | GH43 | ARA86580.1 | Bacillus paralicheniformis MDJK30 | 100 | 533 | 533 | |
| EOOONOAK_01777 | Extracellular endo-alpha-(1- > 5)-l-arabinanase 2 | GH43 | ARA88110.1 | Bacillus paralicheniformis MDJK30 | 99.79 | 469 | 469 | |
| EOOONOAK_02586 | Extracellular endo-alpha-(1- > 5)-l-arabinanase 1 | GH43 | QFY37786.1 | Bacillus paralicheniformis FA6 | 99.69 | 320 | 320 | |
| EOOONOAK_02185 | Arabinoxylan arabinofuranohydrolase | GH43CBM6 | QFY37223.1 | Bacillus paralicheniformis FA6 | 100 | 515 | 515 | |
| EOOONOAK_01157 | hypothetical protein | GH5 | ARA85656.1 | Bacillus paralicheniformis MDJK30 | 99.82 | 560 | 560 | |
| EOOONOAK_02594 | Intracellular exo-alpha-(1- > 5)-l-arabinofuranosidase | GH51 | ARA86758.1 | Bacillus paralicheniformis MDJK30 | 99.80 | 502 | 502 | |
| EOOONOAK_01836 | Arabinogalactan endo-beta-1,4-galactanase | GH53 | AGN38579.1 | Bacillus paralicheniformis ATCC 9945a | 98.58 | 437 | 424 | |
| EOOONOAK_02113 | Levansucrase | GH68 | ARA87315.1 | Bacillus paralicheniformis MDJK30 | 100 | 481 | 481 | |
| EOOONOAK_04229 | Hypothetical protein | GH73 | QEO06161.1 | Bacillus paralicheniformis A4-3 | 98.95 | 570 | 570 | |
| EOOONOAK_03432 | Reducing-end xylose-releasing exo-oligoxylanase Rex8A xylanase probable | GH8 | QFY39429.1 | Bacillus paralicheniformis FA6 | 99.77 | 434 | 434 | |
| Cellulose-related | EOOONOAK_03622 | Endoglucanase S | GH12 | ARA86997.1 | Bacillus paralicheniformis MDJK30 | 100 | 261 | 261 |
| EOOONOAK_01156 | Exoglucanase-2 | GH48 | AJO18128.1 | Bacillus paralicheniformis BL-09 | 100 | 717 | 704 | |
| EOOONOAK_01385 | Endoglucanase | GH5, CBM3 | AJO18373.1 | Bacillus paralicheniformis BL-09 | 99.81 | 518 | 518 | |
| EOOONOAK_01155 | Endoglucanase A | GH9CBM3 | AJO18127.1 | Bacillus paralicheniformis BL-09 | 100 | 653 | 653 | |
| Glucose metabolism | EOOONOAK_00372 | 6-Phospho-beta-glucosidase GmuD | GH1 | AJO18923.1 | Bacillus paralicheniformis BL-09 | 100 | 471 | 471 |
| EOOONOAK_01723 | 6-Phospho-beta-glucosidase GmuD | GH1 | AJO20590.1 | Bacillus paralicheniformis BL-09 | 99.16 | 478 | 478 | |
| EOOONOAK_03054 | Putative 6-phospho-beta-glucosidase | GH4 | ARA84298.1 0 | Bacillus paralicheniformis MDJK30 | 99.55 | 444 | 444 | |
| Hemicellulose or cellulose related | EOOONOAK_01655 | Putative 6-phospho-beta-glucosidase | GH4 | ARA87677.1 | Bacillus paralicheniformis MDJK30 | 100 | 442 | 442 |
| EOOONOAK_01158 | Hypothetical protein | GH5 | QEO07050.1 | Bacillus paralicheniformis A4-3 | 100 | 395 | 395 | |
| Pectin degradation | EOOONOAK_04163 | Unsaturated rhamnogalacturonyl hydrolase YesR | GH105 | AJO17709.1 | Bacillus paralicheniformis BL-09 | 99.71 | 344 | 344 |
| EOOONOAK_02457 | Unsaturated rhamnogalacturonyl hydrolase YteR | GH105 | AJO19596.1 | Bacillus paralicheniformis BL-09 | 99.73 | 373 | 373 | |
| EOOONOAK_02256 | Exo-poly-alpha-d-galacturonosidase | GH28 | QFY37288.1 | Bacillus paralicheniformis FA6 | 100 | 436 | 434 | |
| EOOONOAK_02563 | Hypothetical protein | PL1 | QFY37765.1 | Bacillus paralicheniformis FA6 | 100 | 494 | 494 | |
| EOOONOAK_04252 | Pectate lyase | PL1 | AJO17744.1 | Bacillus paralicheniformis BL-09 | 99.53 | 428 | 428 | |
| EOOONOAK_01685 | Pectate trisaccharide-lyase | PL1 | QEO04986.1] | Bacillus paralicheniformis A4-3 | 99.71 | 341 | 341 | |
| EOOONOAK_04170 | Rhamnogalacturonan exolyase YesX | PL11 | QEO06607.1 | Bacillus paralicheniformis A4-3 | 100 | 628 | 628 | |
| EOOONOAK_04168 | Rhamnogalacturonan endolyase YesW | PL11 | QEO08401.1 | Bacillus paralicheniformis A4-3 | 100 | 622 | 622 | |
| EOOONOAK_04174 | Hypothetical protein | PL26 | QEO06610.1 | Bacillus paralicheniformis A4-3 | 99.07 | 886 | 863 | |
| EOOONOAK_02080 | Pectate lyase C | PL3 | ARA87346.1 | Bacillus paralicheniformis MDJK30 | 99.55 | 222 | 221 | |
| EOOONOAK_01392 | Hypothetical protein | PL9 | QEO07258.1 | Bacillus paralicheniformis A4-3 | 100 | 468 | 468 | |
The accession numbers correspond to the CDS sequences annotated with Prokka as indicated in the manuscript. The corresponding sequences are provided in Figure S2