FIGURE 6.

CS-semi5 downregulates TNF‐α and IL‐1β production and blocks osteoclast differentiation in RAW264.7 cells. (A) RAW264.7 cells were incubated with or without LPS (1 μg/ml) for 24 h. Secretion of TNF‐α and IL‐1β proteins was evaluated by western blot assay. (B,C) Secretion of TNF‐α and IL‐1β proteins was evaluated by ELISA. (D) RAW264.7 cells were cultured for 7 days with RANKL (50 ng/ml) or CS-semi5 (1 μmol/ml), then stained for TRAP activity. Bar indicates 50 μm n = 3. ^# p < 0.05 and ^## p < 0.01 compared with Control Group; *p < 0.05 and **p < 0.01 compared with LPS stimulation Group.