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. 2021 Jul 9;9:707822. doi: 10.3389/fped.2021.707822

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Copyright © 2021 Trisno, Higano, Kechele, Nasr, Chung, Zorn, Woods, Wells and Kingma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological and molecular analysis of the identity of the broncho-esophageal fistula and more distal airway. (A) H&E staining of the patient vs. control tissues from the “proximal” epithelium (broncho-esophageal fistula vs. trachea, respectively), bronchi, and distal bronchioles and alveoli. (B) Immunofluorescence staining of patient tissue for transcription factors SOX2 and NKX2-1 in sections from the proximal epithelium (broncho-esophageal fistula vs. trachea, respectively), bronchi, and distal bronchioles and alveoli. Scale bars represent 50 μm.