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. 2021 Jul 9;22(3):649. doi: 10.3892/ol.2021.12910

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-133a-3p suppresses the proliferation, migration and invasion of CRC cells. DLD-1 and SW480 cells were transfected with miR-133a-3p mimics or mimics NC for 48 h. (A) RT-qPCR results showing the transfection efficiency of miR-133a-3p in transfected DLD-1 and SW480 cells. (B) CCK-8 assay tested the effects of miR-133a-3p mimics on cell proliferation of transfected DLD-1 and SW480 cells. Transwell assay was performed to assess (C) cell migration and (D) invasion in transfected DLD-1 and SW480 cells. HCT116 cells were transfected with miR-133a-3p inhibitor or inhibitor NC for 48 h. (E) RT-qPCR results showing the transfection efficiency of miR-133a-3p in transfected HCT116 cells. (F) CCK-8 assay tested the effects of miR-133a-3p inhibitor on cell proliferation of transfected HCT116 cells. (G) A Transwell assay was performed to assess cell migration and invasion in transfected HCT116 cells. **P<0.01 and ***P<0.001 compared with respective NC. RT-q, reverse transcription-quantitative; miR, microRNA; NC, negative control; CCK-8, Cell Counting Kit-8.