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. 2021 Jul 9;22(3):649. doi: 10.3892/ol.2021.12910

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — AQP1 promotes CRC cell proliferation, migration and invasion. (A) Expression of AQP1 protein was detected in four human CRC cell lines (DLD-1, SW1116, SW480 and HCT116) and a normal colon epithelial cell line (FHC). DLD-1 and SW480 cells were transfected with si-AQP1 or si-NC for 48 h. (B) Expression of AQP1 protein was detected in transfected DLD-1 and SW480 cells using western blot analysis. (C and D) Proliferation of transfected DLD-1 and SW480 cells was assessed by CCK-8 assay. Transwell assay was performed to assess (E) cell migration and (F) invasion in transfected DLD-1 and SW480 cells. (G) Expression of AQP1 protein was detected in HCT116 cells transfected with pcDNA3.1-AQP1 or pcDNA3.1 using western blot analysis. (H) Proliferation of transfected HCT116 cells was assessed using a CCK-8 assay. (I) Transwell assay was performed to assess cell migration and invasion in transfected HCT116 cells. **P<0.01 and ***P<0.001 compared with si-NC or pcDNA3.1. AQP1, aquaporin 1; CCK-8, Cell Counting Kit-8; NC, negative control; si, small interfering.