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. 2021 Jul 9;22(3):649. doi: 10.3892/ol.2021.12910

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Restoration of AQP1 reverses the suppressive effects of miR-133a-3p on CRC cell proliferation, migration and invasion. DLD-1 and SW480 cells were co-transfected with miR-133a-3p mimics and pcDNA3.1-AQP1 or empty pcDNA3.1 for 48 h. (A) Expression of AQP1 protein was detected in transfected DLD-1 and SW480 cells using western blot analysis. (B) Proliferation of transfected DLD-1 and SW480 cells was assessed using a CCK-8 assay. (C) Transwell assay was performed to assess cell migration and invasion in transfected DLD-1 and SW480 cells. **P<0.01 and ***P<0.001 compared with mimics NC + pcDNA3.1. ###P<0.001, compared with miR-133a-3p mimics + pcDNA3.1. AQP1, aquaporin 1; CCK-8, Cell Counting Kit-8; NC, negative control; miR, microRNA.