Figure 5.

Restoration of ATG5-dependent autophagy in DU145 cells enhances apoptosis induced by docetaxel or thapsigargin. (A) ATG5-expressing or vector control cells were treated with the indicated concentration of docetaxel or thapsigargin for 48 h. The number of apoptotic cells was visualized using fluorescent staining with FITC-conjugated Annexin V. ‘Phase’ refers to light microscopic images (magnification, ×100). ‘Annexin V’ refers to images of the FITC-Annexin V fluorescence (magnification, ×100). The FITC-Annexin V fluorescent dots represent the apoptotic cells. (B) Semi-quantification of the apoptotic cells. The data were from three independent experiments. **P<0.01; ***P<0.001. ATG5, autophagy related 5.