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. 2021 Jul 7;22(3):640. doi: 10.3892/ol.2021.12901

Figure 5.

Figure 5.

Expression of the lnc-NLC1-C/miR-383/PRDX-3 axis in U87MG cells and rescue experiments. (A) miR-383 and PRDX-3 expression in U87MG cells was detected using RT-qPCR. (B) PRDX-3 protein expression in U87MG cells was detected using western blotting. (C) miR-383 expression in U87MG glioma cells transfected with miR-383 mimics and miR-383 inhibitors. (D) Expression levels of lnc-NLC1-C, miR-383 and PRDX-3 in rescue experiments were detected using RT-qPCR. (E) PRDX-3 protein expression in rescue experiments was detected using western blotting. **P<0.01; ***P<0.001. n=3. RT-qPCR, reverse transcription-quantitative PCR; lnc-NLC1-C, long non-coding RNA narcolepsy candidate region 1 gene C; ov, overexpression; sh, short hairpin RNA; NC, negative control; miR, microRNA; PRDX-3, peroxiredoxin 3.