FIGURE 4.

Suppressor mutation suppresses targeting defects of partial inner membrane proteins. (A) Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis of the wild-type strain MG1655 and the suppressor strain MY1901. For SEM, the scale bar is 1.0 μm. For TEM, the scale bar is 500 nM. (B) Quantification of identified SRP-dependent inner membrane proteins in strains MY1901 and SRP^– (Supplementary Data Set 1D). Protein abundance of MY1901 and SRP^– is relative to that of wild-type MG1655. (C) FtsQ (left) and EspP (right) targeting assay by their biotinylation. SRP⁺, Ffh expression in HDB51 strain; SRP^–, Ffh depletion in HDB51 strain.