Antibodies |
|
sera from BNT162b2 vaccinated persons |
NCT04743388 |
N/A |
mouse anti-human IgGFc-HRP |
Southern Biotech |
9040-05; RRID:AB_2687484 |
|
Chemicals, peptides, and recombinant proteins |
|
SARS-CoV-2 Spike |
D. Esposito, NCI |
N/A |
|
Critical commercial assays |
|
V-PLEX Human Biomarker Assay kit |
Meso Scale Diagnostics, Maryland, USA |
K15248D-2 |
Elecsys Anti-SARS-CoV-2 S assay |
Roche Diagnostics GmbH, Mannheim, Germany |
N/A |
SARS-CoV-2 Surrogate Virus Neutralization Test |
GenScript, Piscataway, NJ, USA |
N/A |
Nano-Glo® Luciferase Assay System |
Promega, USA |
N1130 |
Luciferase Cell Culture Lysis 5X Reagent |
Promega, USA |
E1531 |
|
Deposited data |
|
Cytokine expression data and analysis code |
This paper |
https://github.com/NCI-VB/felber_covid_vaccine |
|
Experimental models: Cell lines |
|
HEK293T/ACE2wt |
T. Hatziioanou, Rockefeller University |
N/A |
|
Recombinant DNA |
|
pHIVNLDEnv-Nanoluc |
T. Hatziioanou, Rockefeller University |
N/A |
Spike Wuhan1253 |
T. Hatziioanou, Rockefeller University |
N/A |
|
Software and algorithms |
|
GraphPad Prism version 9.0.2 for MacOS X |
GraphPad Software, Inc, La Jolla, CA |
N/A |
R |
this paper |
https://www.r-project.org/ v3.5.1 |
edgeR |
this paper |
https://bioconductor.org/packages/release/bioc/html/edgeR.html v3.24.3 |
limma |
this paper |
https://bioconductor.org/packages/release/bioc/html/limma.html v3.38.3 |
corrplot |
this paper |
https://cran.r-project.org/web/packages/corrplot/index.html v0.84 |
ggpubr |
this paper |
https://cran.r-project.org/web/packages/ggpubr/index.html v0.2.5 |
NIH Integrated Data Analysis Platform |
this paper |
https://nidap.nih.gov/workspace |
Antibody endpoint titer: R functions SSasymp() and nls() from the “stats” R package |
this paper |
https://www.rdocumentation.org/packages/stats/versions/3.6.2/topics/nls |
Cytokine expression data and analysis code |
this paper |
https://github.com/NCI-VB/felber_covid_vaccine |
|
Other |
|
SpectraMax M3 |
Molecular Devices, LLC |
N/A |