Figure 4.

Effect of TCF3 silencing on the expression of Wnt signaling pathway-related genes in G401 kidney tumor cells. (A) Reverse transcription-quantitative PCR was performed to detect the mRNA expression levels of Wnt1, β-catenin and c-myc in all groups. (B) Western blotting was performed to detect the protein expression levels of Wnt1, β-catenin and c-myc in all groups. (C) Semi-quantification of western blotting was performed using ImageJ2 software. Control group, untransfected cells; NC group, cells transfected with NC siRNA; TCF3 siRNA group, cells transfected with TCF3 siRNA. All experiments were repeated at least three times. Data are presented as the mean ± standard error of the mean and were analyzed via one-way ANOVA with Tukey's post hoc test. **P<0.01 and ***P<0.001 compared with the control group; ^##P<0.01 and ^###P<0.001 compared with the NC group. NC, negative control; siRNA, small interfering RNA; TCF3, T-cell factor 3.