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. 2021 Jul 22;16(1):1083–1089. doi: 10.1515/med-2020-0180

Figure 2.

Figure 2

Knockdown of NUSAP1 represses the migration and invasion of MG63 cells under hypoxia. (a) The migration and invasion of MG63 cells whose NUSAP1 expression was knocked down (Hypoxia + KD) under hypoxia condition was measured using transwell assay. (b) The expression of EMT-related proteins was detected using western blot. (c) The expression of MMP family membranes was detected using western blot. All experiments were independently carried out in three replicates. *P < 0.05, **P < 0.01, ***P < 0.001, compared with normoxia group; # P < 0.05, compared with Hypoxia group.