FIGURE 2.

Neutrophils and macrophages do not infiltrate following anti‐ganglioside antibody (AGAb) and complement‐mediated injury to the distal nerve. A, Flow cytometry from mice survived for 3 days (n = 4 Ab + normal human serum (NHS) and NHS, n = 5 naive) showed no differences in neutrophils or macrophage numbers in the diaphragm, nor any large switch to pro‐inflammatory (NOS) or anti‐inflammatory (CD206) phenotype. B, Flow cytometry data were normalised to naïve mouse data. No differences were observed between NHS‐treated and AB + NHS‐treated mice. High variability between mice was observed. C, Illustrative examples of neutrophil immunostaining from mouse diaphragms taken 1 day after NHS. D, Illustrative examples of enhanced green fluorescent protein (eGFP) (eGFP+ve) macrophages in mouse diaphragms taken 3 days following NHS. E, Quantification of neutrophil data shows no significant changes in neutrophil numbers in close proximity to the neuromuscular junction (NMJ). F, Quantification of macrophage numbers showed increases in macrophage numbers, but this was also seen in NHS only treated animals. n = 4/group for immunofluorescence counts * = P < .05, ** = P < .01, one‐way ANOVA with Tukey's multiple comparisons test