Author response image 1. Representative TBM- and TBE-EMSAs assessing parS binding (A and B) and non-specific DNA-binding activity (C), respectively, of Alexa-488-labelled-ParB and its precursor, ParBS68C.

Wild-type-like specific and non-specific DNA-binding activity is retained. In-gel detection of the fluorescent protein corresponds to the pattern of nucleoprotein complexes.