TABLE 2.

The primer sequences for RT-qPCR of cashmere goat gene.

gene Gene	Primer sequence (5′–3′) annealing temperature the sequence of primer Tm (°C)	Fragment length product size
Beta actin	F: GGCAGGTCATCACCATCGG 60	187 bp
	R: CGTGTTGGCGTAGAGGTCTTT
Wnt10b	F: TGCTCACAACCGCAACTC 64	107 bp
	R: GGTCTCGCTCGCAGAAG
Beta-catenin	F: GACCACAAGCAGAGTGCT 55	100 bp
	R: TGTCAGGTGAAGTCCTAAA
SFRP1	F: GCACGACCGTGTGTCCTCCATGT 63	192 bp
	R: GCTTCTTCAGCTCCTTCTTCTTGAT
FGF21	F: TCCCGAAAGTCTCTTGGAGC 57	110 bp
	R: ATCCGTACAGCTTCCCATCTG
TCHHL1	F: GCCAGAAAGTGGCCCAAGATGTAT 58	192 bp
	R: CTCCAAACCATTCTCCTGTCTCAGT

This table shows, the specific primers were designed by Primer 5.0 according to the cDNA sequences of goat Wnt10b, β-catenin, and the actin gene with a constant expression level published in NCBI and were synthesized by Shanghai Biological Engineering Co., Ltd.