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Confocal images of TPP-COOMe (2) and TPP-COOH (3) at 10 μM solution in DMSO (1%) with PC-3 cells after incubation for 1 h at 37 °C. Hoechst nuclear stain was used (1 μg/mL, blue) to visualize the cell nuclei and porphyrins show red fluorescence: (a) Hoechst stained nuclei, porphyrin 2, overlay of blue and red emission channels images; (b) Hoechst stained nuclei, porphyrin 3, overlay of blue and red emission images; Scale bar 20 μm.
