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. Author manuscript; available in PMC: 2021 Jul 23.
Published in final edited form as: Nat Chem Biol. 2020 Aug 10;16(12):1351–1360. doi: 10.1038/s41589-020-0613-y

Extended Data Figure 2: CRISPR-Cas9 genetic screens identify metabolic regulators of the cellular response to cystine depletion and GPX4 inhibition.

Extended Data Figure 2:

(A) Gene scores of Jurkat cells left untreated (x-axis) or cultured in low cystine (10μM, y-axis).

(B) Top-scoring genes under low cystine conditions. Negative scores represent genes whose loss potentiates low cystine toxicity while positive scores represent genes whose loss provides resistance to low cystine.

(C) Gene scores of untreated (x-axis) and erastin treated (3μM, y-axis) Jurkat cells.

(D) Significantly enriched pathways (REACTOME) represented within the top 50 most essential genes in the low dose erastin screen.

(E) Significantly enriched pathways (REACTOME) represented within the top 50 most essential genes in the RSL3 screen.

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