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. 2021 Jul 9;12:691245. doi: 10.3389/fphys.2021.691245

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Copyright © 2021 Krishna, Spaulding, Quindry, Hudson, Quindry and Selsby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Markers of autophagic activation analyzed in a cytosolic fraction from gastrocnemius muscles. Western blot analysis of activation markers of autophagy in a cytosolic fraction from gastrocnemius muscles from 11 month old D2-mdx and DBA mice. (A) ULK1, pULK1 S555, Beclin1and pBeclin1 S91 were decreased, while PI3K III was elevated in D2-mdx gastrocnemius cytoplasmic fraction (CF) compared to DBA. (B) Representative western blot images and corresponding Ponceau stain image. Significance is established at p < 0.05. * Indicates significance between D2-mdx and DBA (n = 7 mice per group).