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. 2021 Jul 23;14:34. doi: 10.1186/s13072-021-00409-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Localization of Nrp1-HA during conjugation stage. A Immunofluorescence staining of Nrp1-HA during conjugation stage. DNA was stained with DAPI. a, pair formation; b, crescent; c, meiosis I; d, meiosis II; e, postzygotic mitosis I; f, postzygotic mitosis II; g, anlagen stgage; h, exconjugant stage. B Co-localizaiton of Nrp1 and α-tubulin during early conjugation stage. The experiments were repeated three times. White arrowheads indicate parental MACs, arrows indicate MICs, stars indicate anlagen, and pounds indicate postzygotic nucleus. Scale bar, 10 µm