Fig. 4.

Micronuclear mitosis and macronuclear amitosis in nrp1i mutants. A Schematic representation for generating NRP1 knockdown mutants. B Identification of NRP1 interference efficiency. Total RNA was isolated from the vegetative growing WT and nrp1i cells. The cells were induced with 0.5 µg/mL Cd²⁺ for 96 h. The relative expression level of NRP1 was identified by qRT-PCR. C Proliferation of nrp1i mutant and WT. D Replicated DNA was labeled with BrdU. Percentage of BrdU-positive (n = 300) in WT cells and nrp1i mutants, respectively. Scale bar, 10 µm. The arrowhead indicates the MAC. E Representative of the division of MIC and MAC. Lost MIC and abnormally divided MAC was showed in the nrp1i mutants. F Statistical analysis of MAC size in WT cells and nrp1i mutants (n = 100). G MIC-specific sequences were amplified by PCR with 10 sets of primers. Primers II to XI were designed for five different chromosomes in MIC, respectively. The loci on chromosomes IV and V were lost in nrp1i mutants. JMJ1 was used as the internal control