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. 2021 Jul 22;9(1):coab058. doi: 10.1093/conphys/coab058

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© The Author(s) 2021. Published by Oxford University Press and the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lumpfish held at atmospheric pressure (grey symbols; 7 per experiment) or exposed to 80 bar of pressure (black symbols; 7 per experiment) were exposed to decreasing (at 2°C h⁻¹; A, C, E) or increasing (at 2°C h⁻¹; B, D, F) temperature in the IPOCAMP chamber. Heart rate (f_H in bpm; A and B) and the percentage change in f_H (as a % of resting values at 0 or 80 bar; C and D) were manually calculated from the ECGs. An asterisk indicates a significant difference between the slopes for relationships between control and pressure-exposed groups. The percentage of QI₀ ECGs (E and F) was provided by the Star-Oddi Mercury software. In these panels, significant differences within the control (regular letters) or pressure-exposed groups (bold letters) are represented by dissimilar lower-case letters and an asterisk indicates a significant difference (P < 0.05) between the pressure-exposed and control groups at a particular temperature step. Data were recorded every 5 minutes, and the symbols represent means ± S.E.M (6 values per fish).